Journal: PLoS ONE

Article Title: Increased Aortic Calpain-1 Activity Mediates Age-Associated Angiotensin II Signaling of Vascular Smooth Muscle Cells

doi: 10.1371/journal.pone.0002231

Figure Lengend Snippet: A. Average calpain-1 transcript abundance from VSMC treated with Ang II for 48 hrs, determined by real time RT-PCR. B. Western blots of calpain-1 of early passage VSMC treated with Ang II for 48 hrs (left panels); average data (right panel). C. Western blots of calpain-1 of early passage VSMC treated with Ang II (10 −7 mol/L) and or SG (10 −7 mol/L), an AT1 antagonist, for 48 hrs (left panels); average data (right panel). D. Western blots of α-II spectrin of early passage VSMC treated with Ang II (10 −7 mol/L) and or SG (10 −7 mol/L), an AT1 antagonist, for 48 hrs (left panels); average data (right panel). *p<0.05, vs. young untreated VSMC (control). #p<0.05, vs. Ang II treated young VSMC. $p<0.05, vs. old untreated VSMC.

Article Snippet: Early passage (p3-p5) VSMC were untreated, or pre-treated with Ang II (100 nmol/L) only or together with [Sar 1 , Gly 8 ]-Angiotensin II acetate hydrate (SG, Cat# A-7401, Sigma, St. Louis, MO) (100 nmol/L), an AT1 blocker, or together with the calpain inhibitor, Ci 1 (100 µmol/L, Cat# 208719, Calbiochem, San Diego, CA) for 48 hours in 0.1% FBS for Western blot and Polyacrylamide Gel Electrophoresis (PAGE) gelatin zymographic analysis.

Techniques: Quantitative RT-PCR, Western Blot

Journal: PLoS ONE

Article Title: Increased Aortic Calpain-1 Activity Mediates Age-Associated Angiotensin II Signaling of Vascular Smooth Muscle Cells

doi: 10.1371/journal.pone.0002231

Figure Lengend Snippet: A. A representative PAGE gelatin zymogram from young and old VSMC treated with Ang II for 48 hrs. B. A representative PAGE gelatin zymogram of VSMC treated with Ang II, or Ci 1, a calpain inhibitor, for 48 hrs (left panel); average data (right panel). C. A representative PAGE gelatin zymogram of VSMC infected adenovirus harboring GFP or CAST treated with Ang II (left panels); average data (right panel). *p<0.05, vs. young untreated VSMC (control). #p<0.05, vs. Ang II treated young VSMC. $p<0.05, vs. old untreated VSMC.

Article Snippet: Early passage (p3-p5) VSMC were untreated, or pre-treated with Ang II (100 nmol/L) only or together with [Sar 1 , Gly 8 ]-Angiotensin II acetate hydrate (SG, Cat# A-7401, Sigma, St. Louis, MO) (100 nmol/L), an AT1 blocker, or together with the calpain inhibitor, Ci 1 (100 µmol/L, Cat# 208719, Calbiochem, San Diego, CA) for 48 hours in 0.1% FBS for Western blot and Polyacrylamide Gel Electrophoresis (PAGE) gelatin zymographic analysis.

Techniques: Infection